Soybean ribulose bisphosphate carboxylase small subunit; mechanisms and determinants of RNA turnover: Annual progress report for the period June 1, 1987 through May 31, 1988
SRS1 and SRS4 are two closely related soybean ribulose -1,5-bisphosphate carboxylase small subunit (SSU) genes. The promoters from both SRS1 and SRS4 can be fused to a neomycin phosphotransferase gene and these fusions produce similar levels of kanamycin resistance in transgenic petunia plants. The expression of SRS1 and SRS4 has been shown to be controlled at the level of transcription, and this transcriptional control is phytochrome mediated. Together these genes account for 2-3% of the total transcription in light-grown soybean seedlings or expanding soybean leaves. Recent experiments analyzing transcription rates of SRS1 and SRS4, steady state levels of their total and poly A+ RNA and frequency of their cDNAs in a soybean RNA library have led us to hypothesize that the expression of these two genes may also be controlled at the level of RNA turnover. Despite the 30-50 fold difference in transcription of these genes in seedlings grown in light, the steady state levels of RNA are only 4-8 fold higher in the light. When plants are shifted from darkness to light, accumulation of RNA lags far behind the striking transcriptional induction. In plants shifted from light to darkness, SRS1 transcription takes 24 hours to drop to dark-grown levels, and the steady state RNA levels take 72 hours to decay to dark-grown levels. On the other hand light-grown plants treated with far-red light shut down SRS1 transcription immediately, and the steady state levels of SSU RNA also drop rapidly. We have evidence suggesting striking differential turnover of the RNA products of SRS1 and SRS4, the SRS1 RNA being perhaps 5-10 times more stable than the SRS4 RNA.
- Research Organization:
- Georgia Univ., Athens (USA). Dept. of Genetics
- DOE Contract Number:
- FG09-86ER13543
- OSTI ID:
- 5323127
- Report Number(s):
- DOE/ER/13543-2; ON: DE88008309
- Country of Publication:
- United States
- Language:
- English
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Differential transcription and message stability of two genes encoding soybean ribulose 1,5-bisphosphate carboxylase small subunit
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
GENE OPERONS
DNA-CLONING
MESSENGER-RNA
BIOLOGICAL HALF-LIFE
RIBULOSE DIPHOSPHATE CARBOXYLASE
GENE REGULATION
RNA PROCESSING
GLYCINE HISPIDA
PROGRESS REPORT
BACTERIA
CARBON-CARBON LYASES
CARBOXY-LYASES
CLONING
DOCUMENT TYPES
ENZYMES
LEGUMINOSAE
LYASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RHIZOBIUM
RNA
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