In vivo studies of biotin absorption in distal rat intestine
The authors have extended their previous studies of biotin absorption in rat proximal jejunum (PJ) to examine biotin absorptive capacity of rat ileum (I) and proximal colon (PC) using in vivo intestinal loop technique. Intestinal loops (2.5 cm) were filled with 0.3 ml of solution containing (/sup 3/H)-biotin and (/sup 14/C)-inulin in phosphate buffer, pH 6.5. Biotin absorption was determined on the basis of luminal biotin disappearance after correction for inulin recovery and averaged (pmol/loop-10 min; X +/- SEM). In related experiments, 5-cm loops of PJ, distal I (DI), or PC were filled with 0.5 ml of solution of similar composition (1.0 ..mu..M biotin). The abdominal cavity was closed and the rats were allowed to recover from anesthesia, then sacrificed 3 hr after injection. Biotin absorption averaged 96.2% (PJ), 93.2% (DI), and 25.8% (PC) of the dose administered. These differences were reflected in the radioactive biotin content of plasma and intestinal loop, kidney, and liver. These data demonstrate significant biotin absorption in rat DI and PC, as required if the intestinal microflora are to be considered as a source of biotin for the host.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 5321140
- Report Number(s):
- CONF-8604222-; TRN: 86-028412
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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BIOTIN
INTESTINAL ABSORPTION
BLOOD PLASMA
CARBON 14 COMPOUNDS
IN VIVO
INTESTINES
INULIN
KIDNEYS
LIVER
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ABSORPTION
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
GLANDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
POLYSACCHARIDES
RODENTS
SACCHARIDES
UPTAKE
VERTEBRATES
VITAMIN B GROUP
VITAMINS
550501* - Metabolism- Tracer Techniques