Total parenteral nutrition in diabetic rats
Parenteral Nutrition with hypertonic glucose is frequently given to diabetic patients. Large amounts of insulin can be required. The purpose of this investigation was to develop a totally parenterally nourished diabetic rat model. 200 g Female Sprague Dawley rats were made diabetic by i.v. injection of streptozotocin (50 mg/kg). Rats were then allowed to recover for at least 1 week before undergoing surgical insertion of a central venous catheter for parenteral feeding. TPN was begun 3 days after surgery. Prior to this they were allowed unlimited access to food and water. Control (non-streptozotocin treated) rats were run at the same time. Protein turnover was investigated by using /sup 15/N glycine. Preliminary results: diabetic rats given mostly fat as a calorie source survived well in the absence of exogenous insulin whereas those that were given glucose only as their non-protein calorie source showed poor survival even with exogenous insulin. N balance and protein turnover in the lipid treated diabetic rats were comparable to the non-diabetic control rats.
- Research Organization:
- Univ. of medicine and Dentistry of New Jersey, Camden
- OSTI ID:
- 5318855
- Report Number(s):
- CONF-8604222-; TRN: 86-028516
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIABETES MELLITUS
BIOLOGICAL MODELS
GLUCOSE
GLYCINE
INSULIN
NITROGEN 15
NUTRITION
RATS
SURVIVAL TIME
TRACER TECHNIQUES
ALDEHYDES
AMINO ACIDS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACIDS
DISEASES
ENDOCRINE DISEASES
HEXOSES
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METABOLIC DISEASES
MONOSACCHARIDES
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
RODENTS
SACCHARIDES
STABLE ISOTOPES
VERTEBRATES
550901* - Pathology- Tracer Techniques