Identification of rat brain opioid (enkephalin) receptor by photoaffinity labeling
Journal Article
·
· NIDA Res. Monogr.; (United States)
OSTI ID:5302755
A photoreactive, radioactive enkephalin derivative was prepared and purified by high performance liquid chromatography. Rat brain and spinal cord plasma membranes were incubated with this radioiodinated photoprobe and were subsequently photolysed. Autoradiography of the sodium dodecyl sulfate gel electrophoresis of the solubilized and reduced membranes showed that a protein having an apparent molecular weight of 46,000 daltons was specifically labeled, suggesting that this protein may be the opioid (enkephalin) receptor.
- Research Organization:
- Univ. of Toronto, Ontario (Canada)
- OSTI ID:
- 5302755
- Journal Information:
- NIDA Res. Monogr.; (United States), Vol. 75
- Country of Publication:
- United States
- Language:
- English
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+5 more
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ENKEPHALINS
RECEPTORS
LABELLING
AUTORADIOGRAPHY
BRAIN
CELL MEMBRANES
ELECTROPHORESIS
IODINE ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MOLECULAR WEIGHT
RATS
SPINAL CORD
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DRUGS
ENDORPHINS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RODENTS
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
ENKEPHALINS
RECEPTORS
LABELLING
AUTORADIOGRAPHY
BRAIN
CELL MEMBRANES
ELECTROPHORESIS
IODINE ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MOLECULAR WEIGHT
RATS
SPINAL CORD
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DRUGS
ENDORPHINS
ISOTOPES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RODENTS
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques