A photoactivable phospholipid analogue that specifically labels membrane cytoskeletal proteins of intact erythrocytes

Journal Article · Tue Aug 22 00:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00443a025· OSTI ID:5300479

Pradhan, D; Williamson, P; Schlegel, R A ^[1]

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1. Pennsylvania State Univ., University Park (USA)

A radioactive photoactivable analogue of phosphatidylethanolamine, 2-(2-azido-4-nitrobenzoyl)-1-acyl-sn-glycero-3-phospho({sup 14}C)ethanolamine(({sup 14}C)AzPE), was synthesized. Upon incubation with erythrocytes in the dark, about 90% of ({sup 14}C)AzPE spontaneously incorporated into the cells; of this fraction, about 90% associated with the membrane, all of it noncovalently. Upon photoactivation, 3-4% of the membrane-associated probe was incorporated into protein. Analysis of this fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as well as extraction of labeled membranes with alkali or detergent, showed that the probe preferentially labeled cytoskeletal proteins. ({sup 14}C)AzPE appears to be a useful tool for the study of lipid-protein interactions at the cytoplasmic face of the plasma membrane of intact cells.

• OSTI ID: 5300479
• Journal Information: Biochemistry; (USA), Vol. 28:17; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ERYTHROCYTES
CROSS-LINKING
MEMBRANE PROTEINS
LABELLING
PHOSPHOLIPIDS
PROTEINS
AMINES
CARBON 14 COMPOUNDS
CELL MEMBRANES
MEMBRANES
PHOTOLYSIS
THIN-LAYER CHROMATOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
ESTERS
LABELLED COMPOUNDS
LIPIDS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOTOCHEMICAL REACTIONS
POLYMERIZATION
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques