SANS studies of interacting hemoglobin in intact erythrocytes
Small angle neutron scattering (SANS) was used to investigate interaction forces between hemoglobin (Hb) molecules contained within human red cells. The scattering separately attributable to cell membranes and intracellular Hb was identified. A series of D/sub 2/O-H/sub 2/O contrast variation measurements were made in order to establish conditions for which scattering from the cell membrane is minimized (approximately 15% D/sub 2/O). Measurements then were performed to examine changes in intermolecular Hb interactions occurring when the cells are contracted or swollen by varying the ionic strength of the suspension buffer. The scattering cross-sections were fitted to structure factors computed by a mean spherical approximation, and molecular parameters thereby extracted. Oxygenation studies on normal cells were performed, and results contrasted with those of similar studies of erythrocytes obtained from sickle cell disease patients.
- Research Organization:
- Univ. of Maryland, College Park (USA)
- OSTI ID:
- 5298993
- Journal Information:
- Biophys. J.; (United States), Vol. 53:1
- Country of Publication:
- United States
- Language:
- English
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HEMOGLOBIN
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
DEUTERIUM
ERYTHROCYTES
HEAVY WATER
NEUTRONS
SCATTERING
SICKLE CELL ANEMIA
TRACER TECHNIQUES
ANEMIAS
BARYONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DISEASES
ELEMENTARY PARTICLES
FERMIONS
GLOBIN
HADRONS
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MATERIALS
MEMBRANES
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
REACTION KINETICS
STABLE ISOTOPES
SYMPTOMS
WATER
550201* - Biochemistry- Tracer Techniques