2,3,7,8-tetrachlorodibenzo-p-dioxin: examination of biochemical effects involved in the proliferation and differentiation of XB cells

Journal Article · Mon Oct 01 00:00:00 EDT 1984 · J. Cell. Physiol.; (United States)

DOI:https://doi.org/10.1002/jcp.1041210118· OSTI ID:5275523

Knutson, J C; Poland, A

XB, a cell line derived form a mouse teratoma, differentiates into stratified squamous epithelium when incubated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). To examine the mediators of this response the effects produced by TCDD and those elicited by other compounds which stimulated epidermal proliferation and/or differentiation in mice were compared, XB/3T3 cultures keratinize when incubated with cholera toxin, epidermal growth factor (EGF), or TCDD , but not 12-0-tetradecanoylphorbol-13-acetate (TPA). Incubation of XB cells with TCDD for 48 hours produces an increase in thymidine incorporation, a response which is neither as large nor as rapid as that produced by cholera toxin, TPA, or EGF. Although both cholera toxin and TCDD stimulate differentiation and thymidine incorporation in XB/3T3 cultures, cholera toxin increases cAMP 30-fold in these cells, while TCDD does not affect cAMP accumulation. Inhibitors of arachidonic acid metabolism, which block epidermal proliferative responses to TPA in vivo, do not prevent the differentiation of XB cells in response to TCDD. In XB/3T3 cultures, TPA stimulates arachidonic acid release at all times tested (1,6, and 24 hours) and increases the incorporation of /sup 32/P/sub i/ into total phospholipids and phosphatidyl-choline after 3 hours. In contrast, D affects neither arachidonic acid release nor the turnover of phosphatidylinositol, or phosphatidylcholine at any of the times tested. Although biochemical effects which have been suggested as part of the mechanism of TCDD and produced by other epidermal proliferative compounds in XB cells were examined, no mediator of the TCDD-produced differentiation of XB/3T3 cultures was observed.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5275523
• Journal Information: J. Cell. Physiol.; (United States), Vol. 121:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CHLORINATED AROMATIC HYDROCARBONS
BIOCHEMISTRY
PHOSPHORUS 32
UPTAKE
TUMOR CELLS
CELL DIFFERENTIATION
CELL PROLIFERATION
METABOLISM
SKIN
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
AROMATICS
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BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHEMISTRY
DAYS LIVING RADIOISOTOPES
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
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