Characterization of a panel of somatic cell hybrids for regional mapping of the mouse X chromosome
A panel of five hybrid cell lines containing mouse X chromosomes with various deletions has been obtained by fusing splenocytes from male mice carrying one of a series of reciprocal X-autosome translocations with the azaguanine-resistant Chinese hamster cell line CH3g. These hybrids have been extensively characterized by using the allozymes hypoxanthine/guanine phosphoribosyltransferase (encoded by the Hprt locus) and ..cap alpha..-galactosidase (Ags) and a series of 11 X-chromosome-specific DNA probes whose localization had been previously established by linkage studies. Such studies have established the genetic breakpoints of the T(X;12)13R1 and T(X;2)14R1 X-autosome translocations on the X chromosome and provided additional information as to the X-chromosome genetic breakpoints of the T(X;16)16H, T(X;4)7R1, and T(X;7)6R1 translocations. The data establish clearly that both the T(X;7)5RI and T(X;12)13R1 X-chromosome breakpoints are proximal to Hprt, the breakpoint of the former being more centromeric, lying as it does in the 9-centimorgan interval between the ornithine transcarbamoylase (Otc) and DXPas7 (M2C) loci. These five hybrid cell lines provide, with the previously characterized EBS4 hybrid cell line, a nested series of seven mapping intervals distributed along the length of the mouse X chromosome. Their characterization not only allows further correlation of the genetic and cytological X-chromosome maps but also should permit the rapid identification of DNA probes specific for particular regions of the mouse X chromosome.
- Research Organization:
- Institut Pasteur, Paris (France)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 5239169
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:15
- Country of Publication:
- United States
- Language:
- English
Similar Records
Physical mapping of the split hand/split foot (SHSF) locus on chromosome 7 reveals a relationship between SHSF and the syndromic ectrodactylies
Measurement of transcribed human X-chromosomal DNA sequences transferred to rodent cells by chromosome-mediated gene transfer
Related Subjects
DNA
HYBRIDIZATION
X CHROMOSOME
GENETIC MAPPING
CHROMOSOMAL ABERRATIONS
FIBROBLASTS
GUANINE
HYPOXANTHINE
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
MICE
SPLEEN CELLS
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
CHROMOSOMES
CONNECTIVE TISSUE CELLS
ENZYMES
GLYCOSYL TRANSFERASES
HETEROCHROMOSOMES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MAPPING
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSYL TRANSFERASES
PURINES
RODENTS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES
550401* - Genetics- Tracer Techniques