Immobilization of BSA, enzymes and cells of Bacillus stearothermophilus onto cellulose polygalacturonic acid and starch based graft copolymers containing maleic arhydride
Poly(maleic anhydride styrene) graft copolymers of cellulose, pectin polygalacturonic acid salt, calcium polygalacturonate, and starch were prepared and used to immobilize proteins. The cellulose grafts coupled quite appreciable quantities of acid phosphatase, glucose oxidase, and trypsin. However, the general retention of activity was somewhat disappointing. Further investigation with acid phosphatase showed that the amount of enzyme immobilized increased as the amount of anhydride in the graft copolymer increased but no such relationship existed for the enzymic activity. The cellulose graft copolymers were hydrolyzed and it appeared that the carboxyl group aided adsorption of the enzyme. Attempts to couple acid phosphatase using CMC through the free carboxyl groups, created by hydrolysis, gave only a small increase in the extent of protein coupling. However, the unhydrolyzed system gave a useful degree of immobilization of cells of Bacillus stearothermophilus, as did a poly(maleic anhydride/styrene)-cocellulose system. Attempts to improve the activity by using grafts based on other polysaccharide supports met with mixed success. Pectin products were soluble. Polygalacturonic acid products were partially soluble and extremely high levels of enzymic activity were obtained. This was probably due in part to the hydrophilic nature of the system, which also encouraged absorption of the enzyme. Attempts were made to reduce the solubility by using the calcium pectinate salt. Immobilization of acid phosphatase and trypsin resulted in increased protein coupling but relatively poor activities were attained. Calcium polygalacturonate was used to prepare an insoluble graft copolymeric system containing acrylonitrile-comaleic anhydride. The resulting gels gave excellent coupling with acid phosphatase which had a very good retention of activity.
- Research Organization:
- Leeds Polytechnic, England; Univ. of Coimbra, Portugal; Leeds Univ., England
- OSTI ID:
- 5204729
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 28:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
BACILLUS
IMMOBILIZED CELLS
CHEMICAL PREPARATION
IMMOBILIZED ENZYMES
OXIDOREDUCTASES
PHOSPHATASES
ADSORPTION
ALBUMINS
CALCIUM
CATTLE
CELLULOSE
ENZYME ACTIVITY
GALACTURONIC ACID
GELS
HYDROLYSIS
PECTINS
POLYMERS
POLYSTYRENE
PROTEINS
SALTS
SOLUBILITY
STARCH
ALDEHYDES
ALKALINE EARTH METALS
ANIMALS
BACTERIA
BLOOD SUBSTITUTES
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
COLLOIDS
DECOMPOSITION
DISPERSIONS
DOMESTIC ANIMALS
DRUGS
ELEMENTS
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HYDROLASES
HYDROXY ACIDS
LYSIS
MAMMALS
MATERIALS
METALS
MICROORGANISMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLYOLEFINS
POLYSACCHARIDES
POLYVINYLS
REAGENTS
RUMINANTS
SACCHARIDES
SOLVOLYSIS
SORPTION
SYNTHESIS
SYNTHETIC MATERIALS
VERTEBRATES
550700* - Microbiology
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)