Glutathione (GSH) production is increased in copper deficient isolated hepatocytes and inhibition of GSH synthesis decreases intracellular cholesterol

Conference · Fri Mar 15 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5202679

Chao, Pi Yu; Allen, K G D ^[1]

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1. Colorado State Univ., Ft. Collins (United States)

Thirty male weanling Sprague Dawley rats were assigned to three groups of 10 animals each and fed AIN based copper deficient ad libitum, copper control meal fed and copper deficient meal fed diets. 12h meals were provided. Beginning on day 54 animals were used to prepare isolated hepatocytes, following a 4h meal, by portal vein collagenase infusion. Washed isolated hepatocytes were incubated in Krebs Henseleit bicarbonate buffer pH 7.4 under 95/5 O{sub 2}/CO{sub 2} at 37{degree} with 2.5 mM Ca, 0.75% gelatin and 10 mM glucose, either with or without 1 mM buthionine sulfoximine (BSO) a specific inhibitor of GSH synthesis. Hepatocyte viability was assessed by trypan blue exclusion. Copper deficiency significantly increased GSH production by 30-50% at 1.5 and 3 h, with no changes in GSSG. GSH production was inhibited by BSO which also significantly inhibited intracellular cholesterol appearance at 3 h. The intracellular (GHS){sup 2}/GSSG at 3 hr showed a significant correlation with cholesterol appearance rate at 3 h. Copper deficiency increases hepatocyte GSH production. Inhibition of GSH production is correlated with decreased cholesterol appearance.

• OSTI ID: 5202679
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHOLESTEROL
BIOSYNTHESIS
COPPER
BIOLOGICAL EFFECTS
GLUTATHIONE
ENZYME INHIBITORS
INHIBITION
LIVER CELLS
METABOLISM
NUTRITIONAL DEFICIENCY
RATS
ANIMAL CELLS
ANIMALS
DRUGS
ELEMENTS
HYDROXY COMPOUNDS
MAMMALS
METALS
ORGANIC COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
SOMATIC CELLS
STEROIDS
STEROLS
SYNTHESIS
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology