DNA damage and biological expression of adenovirus: A comparison of liquid versus frozen conditions of exposure to gamma rays
- McMaster Univ., Hamilton, Ontario (Canada)
Human adenovirus type 2 (Ad 2) was irradiated with 137Cs gamma rays in the liquid state at 0 degree C. DNA breaks were correlated with the inactivation of several viral functions and compared to results obtained previously for irradiation of Ad 2 under frozen conditions at -75 degrees C. Irradiation at 0 degree C induced 170 +/- 20 single-strand breaks and 2.6 +/- 0.4 double-strand breaks/Gy/10(12) Da in the viral DNA. Viral adsorption to human KB cells was inactivated with a D0 of 9.72 +/- 1.18 kGy, whereas the inactivation of Ad 2 plaque formation had a D0 of 0.99 +/- 0.14 or 1.1 +/- 0.29 kGy when corrected for the effect of radiation on virus adsorption. For the adsorbed virus, an average of 4.3 +/- 1.7 single-strand and 0.065 +/- 0.02 double-strand breaks were induced in the viral DNA per lethal hit. In contrast, irradiation of Ad 2 at -75 degrees C results in 2.6- to 3.4-fold less DNA breakage per Gy and a 5.6-fold increase in D0 for plaque formation of the adsorbed virus. Furthermore, although host cell reactivation (HCR) of Ad 2 viral structural antigen production for irradiated virus was substantially reduced in the xeroderma pigmentosum fibroblast strain (XP25RO) compared to normal strains for irradiation at -75 degrees C (57% HCR), it was only slightly reduced compared to normal for irradiation at 0 degree C (88% HCR). These results indicate that the spectrum of DNA damage is both quantitatively and qualitatively different for the two conditions of irradiation.
- OSTI ID:
- 5183996
- Journal Information:
- Radiation Research; (USA), Vol. 120:1; ISSN 0033-7587
- Country of Publication:
- United States
- Language:
- English
Similar Records
Host cell reactivation of sunlamp-exposed adenovirus in fibroblasts from patients with Bloom's syndrome, ataxia telangiectasia, and Huntington's disease
Relationships among cell survival, O6-alkylguanine-DNA alkyltransferase activity, and reactivation of methylated adenovirus 5 and herpes simplex virus type 1 in human melanoma cell lines
Related Subjects
ADENOVIRUS
GENETIC RADIATION EFFECTS
STRAND BREAKS
RADIOINDUCTION
CESIUM 137
DNA
DNA REPAIR
FREEZING
GAMMA RADIATION
MAN
TEMPERATURE DEPENDENCE
XP CELLS
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CESIUM ISOTOPES
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
VERTEBRATES
VIRUSES
YEARS LIVING RADIOISOTOPES
560130* - Radiation Effects on Microorganisms