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Title: The effects of retinoic acid on immunoglobulin synthesis: Role of interleukin 6

Abstract

Retinoic acid (RA) and its parent compound, retinol (ROH, vitamin A), have been recognized as important immunopotentiating agents. Previous studies from our laboratory have demonstrated that PA can augment formalin-treated Staphylococcus aureus (SAC) stimulated immunoglobulin (Ig) synthesis of cord blood mononuclear cells (CBMC). To determine the mechanism(s) by which RA modulates Ig synthesis, we studied the effects of RA on B cells and cytokine production. The addition of RA (10{sup -5} to 10{sup -10} M) to Epstein-Barr virus (EBV)-transformed B-cell clones derived from either adult or cord blood B cells augmented Ig secretion twofold. In contrast, cell proliferation was inhibited as measured by {sup 3}H-thymidine incorporation. We evaluated two cytokines known to be constitutively produced by EBV cell lines, IL-1 and IL-6. While RA had no effect on IL-1 production, IL-6 synthesis was greatly enhanced (20- to 45-fold), which was also reflected by an increase in steady-state mRNA levels for IL-6 but not TNF-{alpha} or TGF-{beta} on Northern blot analysis. Polyclonal rabbit anti-IL-6 antibodies were used to block the augmenting effects of RA on Ig synthesis of adenoidal B cells. RA-induced augmentation in IgG and IgA synthesis was blocked 58 and 29%, respectively, by anti-IL-6 antibodies. These studies suggest thatmore » the enhancing effects of RA on Ig synthesis are mediated, at least in part, by the autocrine or paracrine effects of IL-6 on B-cell differentiation. 37 refs., 5 figs.« less

Authors:
; ; ;  [1]
  1. Children`s Hospital of Buffalo, NY (United States)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
518334
Resource Type:
Journal Article
Journal Name:
Journal of Clinical Immunology
Additional Journal Information:
Journal Volume: 16; Journal Issue: 3; Other Information: PBD: May 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ANIMAL CELLS; CELL PROLIFERATION; CLONING; RETINOIC ACID; BIOLOGICAL EFFECTS; IMMUNOGLOBULINS; BIOSYNTHESIS; VITAMIN A; IMMUNE REACTIONS; LYMPHOKINES; ANTIBODIES

Citation Formats

Ballow, M, Xiang, Shunan, Wang, Weiping, Brodsky, L, and State Univ. of New York, Buffalo, NY. The effects of retinoic acid on immunoglobulin synthesis: Role of interleukin 6. United States: N. p., 1996. Web. doi:10.1007/BF01540916.
Ballow, M, Xiang, Shunan, Wang, Weiping, Brodsky, L, & State Univ. of New York, Buffalo, NY. The effects of retinoic acid on immunoglobulin synthesis: Role of interleukin 6. United States. https://doi.org/10.1007/BF01540916
Ballow, M, Xiang, Shunan, Wang, Weiping, Brodsky, L, and State Univ. of New York, Buffalo, NY. 1996. "The effects of retinoic acid on immunoglobulin synthesis: Role of interleukin 6". United States. https://doi.org/10.1007/BF01540916.
@article{osti_518334,
title = {The effects of retinoic acid on immunoglobulin synthesis: Role of interleukin 6},
author = {Ballow, M and Xiang, Shunan and Wang, Weiping and Brodsky, L and State Univ. of New York, Buffalo, NY},
abstractNote = {Retinoic acid (RA) and its parent compound, retinol (ROH, vitamin A), have been recognized as important immunopotentiating agents. Previous studies from our laboratory have demonstrated that PA can augment formalin-treated Staphylococcus aureus (SAC) stimulated immunoglobulin (Ig) synthesis of cord blood mononuclear cells (CBMC). To determine the mechanism(s) by which RA modulates Ig synthesis, we studied the effects of RA on B cells and cytokine production. The addition of RA (10{sup -5} to 10{sup -10} M) to Epstein-Barr virus (EBV)-transformed B-cell clones derived from either adult or cord blood B cells augmented Ig secretion twofold. In contrast, cell proliferation was inhibited as measured by {sup 3}H-thymidine incorporation. We evaluated two cytokines known to be constitutively produced by EBV cell lines, IL-1 and IL-6. While RA had no effect on IL-1 production, IL-6 synthesis was greatly enhanced (20- to 45-fold), which was also reflected by an increase in steady-state mRNA levels for IL-6 but not TNF-{alpha} or TGF-{beta} on Northern blot analysis. Polyclonal rabbit anti-IL-6 antibodies were used to block the augmenting effects of RA on Ig synthesis of adenoidal B cells. RA-induced augmentation in IgG and IgA synthesis was blocked 58 and 29%, respectively, by anti-IL-6 antibodies. These studies suggest that the enhancing effects of RA on Ig synthesis are mediated, at least in part, by the autocrine or paracrine effects of IL-6 on B-cell differentiation. 37 refs., 5 figs.},
doi = {10.1007/BF01540916},
url = {https://www.osti.gov/biblio/518334}, journal = {Journal of Clinical Immunology},
number = 3,
volume = 16,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1996},
month = {Wed May 01 00:00:00 EDT 1996}
}