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Title: Purification and characterization of a proteasome from the hyperthermophilic archaeon pyrococcus furiosus

Journal Article · · Applied and Environmental Microbiology
OSTI ID:518324
; ;  [1]
  1. North Carolina State Univ., Raleigh, NC (United States)

A 640-kDa proteasome consisting of {alpha} (25-kDa) and {beta} (22-kDa) subunits, and with a temperature optimum of 95{degrees}C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota. 38 refs., 5 figs., 2 tabs.

OSTI ID:
518324
Journal Information:
Applied and Environmental Microbiology, Vol. 63, Issue 3; Other Information: PBD: Mar 1997
Country of Publication:
United States
Language:
English