Reverse genetics: Its origins and prospects

Journal Article · Mon Apr 01 00:00:00 EST 1991 · Bio/Technology; (United States)

DOI:https://doi.org/10.1038/nbt0491-342· OSTI ID:5176283

Berg, P ^[1]

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1. Stanford Univ., CA (United States)

The nucleotide sequence of a gene and its flanking segments alone will not tell us how its expression is regulated during development and differentiation, or in response to environmental changes. To comprehend the physiological significance of the molecular details requires biological analysis. Recombinant DNA techniques provide a powerful experimental approach. A strategy termed reverse genetics' utilizes the analysis of the activities of mutant and normal genes and experimentally constructed mutants to explore the relationship between gene structure and function thereby helping elucidate the relationship between genotype and phenotype.

• OSTI ID: 5176283
• Journal Information: Bio/Technology; (United States), Vol. 9:4; ISSN 0733-222X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE REGULATION
MOLECULAR BIOLOGY
BACTERIOPHAGES
CHROMOSOMES
DNA SEQUENCING
DROSOPHILA
ESCHERICHIA COLI
GENETICS
GENOTYPE
MAMMALS
MUTATIONS
NEMATODES
PHENOTYPE
YEASTS
ANIMALS
ARTHROPODS
ASCHELMINTHES
BACTERIA
BIOLOGY
DIPTERA
EUMYCOTA
FLIES
FRUIT FLIES
FUNGI
HELMINTHS
INSECTS
INVERTEBRATES
MICROORGANISMS
PARASITES
PLANTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
VIRUSES
550400* - Genetics
550200 - Biochemistry