Regulation of beta follicle stimulating hormone subunit RNA by 17-beta estradiol, progesterone, and inhibin in ovine pituitary cells in culture

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5174912

Phillips, C L

The molecular mechanism by which ovine follicle stimulating hormone (FSH) is negatively regulated by 17-beta estradiol, progesterone, and inhibin was investigated in vitro, using ovine pituitary cells in culture. The effects of these gonadal hormones on beta FSH RNA levels were assayed by dot blot hybridization to a specific radiolabeled cDNA probe for beta FSH RNA. This was compared to concomitant changes in FSH secretion, which were measured by radioimmunoassay, in order to determine if the alterations in beta FSH RNA could account for the changes in FSH secretion.

• Research Organization: North Carolina State Univ., Raleigh (USA)
• OSTI ID: 5174912
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESTRADIOL
BIOLOGICAL FUNCTIONS
FSH
BIOSYNTHESIS
RADIOIMMUNOASSAY
PROGESTERONE
CELL CULTURES
PITUITARY GLAND
RNA
BODY
ENDOCRINE GLANDS
ESTRANES
ESTROGENS
FUNCTIONS
GLANDS
GONADOTROPINS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KETONES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
RADIOASSAY
RADIOIMMUNOLOGY
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques