Respective roles of pyrimidine dimer and pyrimidine (6-4) pyrimidone photoproducts in UV mutagenesis of simian virus 40 DNA in mammalian cells

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Journal of Virology; (USA)

OSTI ID:5156387

Bourre, F; Benoit, A; Sarasin, A ^[1]

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1. Institut de Recherches Scientifiques sur le Cancer, Villejuif (France)

UV light induces DNA lesions which are mutagenic in mammalian cells. We used simian virus 40 tsB201 (unable to produce viral capsid at the restrictive temperature of 41{degree}C because of a point mutation in the VP1 gene) to analyze the mutagenic potency of the two major UV-induced lesions, pyrimidine dimers (Py-Py) and pyrimidine (6-4) pyrimidones (Py(6-4)Py), which are formed on the same nucleotide sites. The mutagenesis criterion was the reversion toward a wild-type growth phenotype. After UV irradiation (mainly at 254 nm), part of the DNA was treated with the photoreactivating enzyme of Escherichia coli, which monomerizes Py-Py but does not modify the Py(6-4)Py photoproduct. Higher survival and lower mutation frequency rates for the photoreactivated DNA indicated that the two lesions were lethal and mutagenic. The VP1 gene of some mutants was entirely sequenced. The mutation spectra showed that the two lesions did not induce the same mutation hot spots, although some sites were common to both. The induced mutation hot spots were not only correlated with lesion hot spots but seemed partially directed by local DNA structures.

• OSTI ID: 5156387
• Journal Information: Journal of Virology; (USA), Vol. 63:11; ISSN 0022-538X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GENE MUTATIONS
RADIOINDUCTION
SIMIAN VIRUS
GENETIC RADIATION EFFECTS
DNA
GENES
MUTAGENESIS
MUTATION FREQUENCY
PHENOTYPE
PYRIMIDINE DIMERS
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
RADIATION EFFECTS
RADIATIONS
VIRUSES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture