Preliminary studies on the activities of spin traps as scavengers of free radicals

Conference · Fri Mar 15 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5145220

Ogunbiyi, P O; Washington, I ^[1]

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1. Tuskegee Univ., AL (United States)

The spin trapping agents, N-t-Butyl-a-phenyl-nitrone (PBN) and 5,5-Dimethyl-1-pyroline-N-oxide (DMPO) have been used to investigate the primary free radicals involved in various tissue injuries. Also, PBN and DMPO can provide some protection against free radical-induced lung injuries. However, their therapeutic potentials as free radical scavengers remained unexamined. In this study, the effects of PBN and DMPO on guinea pig lung microsomal lipid peroxidation were investigated using thiobarbituric acid-reactive substance assay. Superoxide anions (O{sup 2}{minus}) were generated in an enzymatic and a non-enzymatic system. PBN and DMPO each, significantly inhibited NADPH-stimulated lipid peroxidation irrespective of the presence of Fe{sup 3+}. Cytochrome c reduction by the enzymatic and nitro blue tetrazolium reduction by the non-enzymatic O{sup 2}{minus} generating systems were both inhibited by PBN and DMPO as well as superoxide dismutase and dimethyl sulfoxide when compared with the controls. The spin traps exhibited lower potencies in these systems than the reference compounds, SOD and DMSO, which are well established as O{sup 2}{minus} and hydroxyl radical scavengers respectively. Results demonstrate the free radical scavenging properties of PBN and DMPO. This is an indication of their possible usefulness as antioxidants.

• OSTI ID: 5145220
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIOXIDANTS
CHEMICAL PROPERTIES
LIPIDS
METABOLISM
RADICALS
TOXICITY
ELECTRON SPIN RESONANCE
GUINEA PIGS
LUNGS
MICROSOMES
SUPEROXIDE DISMUTASE
ANIMALS
BODY
CELL CONSTITUENTS
ENZYMES
MAGNETIC RESONANCE
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PROTEINS
RESONANCE
RESPIRATORY SYSTEM
RIBOSOMES
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology