Quantitation of water in membranes by neutron diffraction and X-ray techniques

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(86)27018-4· OSTI ID:5130689

Knott, R B; Schoenborn, B P

The general principle of placing neutron and X-ray scattering density profiles on an absolute scale is being applied to an increasing number of problems in structural biology. This maximizes the information from the experiments by facilitating the identification of various molecular species. The greater detail available on the membrane water distribution has been highlighted in this chapter. The quantitative analysis of water in the headgroup region and the intermembrane water layer provides valuable information on membrane structure and function. The single most important limitation of the method is the lack of resolution. Improvements in experimental techniques will improve the resolution in a number of situations.

• OSTI ID: 5130689
• Journal Information: Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CELL MEMBRANES
NEUTRON DIFFRACTION
X-RAY DIFFRACTION
WATER
QUANTITATIVE CHEMICAL ANALYSIS
CELL CONSTITUENTS
CHEMICAL ANALYSIS
COHERENT SCATTERING
DIFFRACTION
HYDROGEN COMPOUNDS
MEMBRANES
OXYGEN COMPOUNDS
SCATTERING
500200* - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)
400101 - Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures