DNA sequence, structure, and tyrosine kinase activity of the Drosophila melanogaster abelson proto-oncogene homolog
The authors report their molecular characterization of the Drosophila melanogaster Abelson gene (abl), a gene in which recessive loss-of-function mutations result in lethality at the pupal stage of development. This essential gene consists of 10 exons extending over 26 kilobase pairs of genomic DNA. The DNA sequence encodes a protein of 1,520 amino acids with strong sequence similarity to the human c-abl proto-oncogene beginning in the type 1b 5' exon and extending through the region essential for tyrosine kinase activity. When the tyrosine kinase homologous region was expressed in Escherichia coli, phosphorylation of proteins on tyrosine residues was observed with an antiphosphotyrosine antibody. These results show that the abl gene is highly conserved through evolution and encodes a functional tyrosine protein kinase required for Drosophila development.
- Research Organization:
- McArdle Lab. for Cancer Research, Univ. of Wisconsin, Madison, WI 53706 (US)
- OSTI ID:
- 5113146
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DROSOPHILA
GENETIC MAPPING
MUTATIONS
ONCOGENES
STRUCTURE-ACTIVITY RELATIONSHIPS
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
AMINO ACID SEQUENCE
ANTIBODIES
DNA SEQUENCING
GENE RECOMBINATION PROTEINS
TYROSINE
AMINO ACIDS
ANIMALS
ARTHROPODS
CARBOXYLIC ACIDS
DIPTERA
ENZYMES
FLIES
FRUIT FLIES
GENES
HYDROXY ACIDS
INSECTS
INVERTEBRATES
MAPPING
MOLECULAR STRUCTURE
NUCLEOPROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550400* - Genetics