Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5105184

Alterthum, F ^[1]; Ingram, L O ^[2]

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1. Univ. de Sao Paulo (Brazil)
2. Univ. of Florida, Gainesville (USA)

Lactose and all of the major sugars (glucose, xylose, arabinose, galactose, and mannose) present in cellulose and hemicellulose were converted to ethanol by recombinant Escherichia coli containing plasmid-borne genes encoding the enzymes for the ethanol pathway from Zymomonas mobilis. Environmental tolerances, plasmid stability, expression of Z. mobilis pyruvate decarboxylase, substrate range, and ethanol production (from glucose, lactose, and xylose) were compared among eight American Type Culture Collection strains. E. coli ATCC 9637(pLOI297), ATCC 11303(pLOI297), and ATCC 15224(pLOI297) were selected for further development on the basis of environmental hardiness and ethanol production. Volumetric ethanol productivities per hour in batch culture were 1.4 g/liter for glucose (12%), 1.3 g/liter for lactose (12%), and 0.64 g/liter for xylose (8%). Ethanol productivities per hour ranged from 2.1 g/g of cell dry weight with 12% glucose to 1.3 g/g of cell dry weight with 8% xylose. The ethanol yield per gram of xylose was higher for recombinant E. coli than commonly reported for Saccharomyces cerevisiae with glucose. Glucose (12%), lactose (12%), and xylose (8%) were converted to (by volume) 7.2% ethanol, 6.5% ethanol, and 5.2% ethanol, respectively.

• OSTI ID: 5105184
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 55:8; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ESCHERICHIA COLI
PRODUCTIVITY
ETHANOL
BIOSYNTHESIS
GLUCOSE
FERMENTATION
LACTOSE
XYLOSE
COMPARATIVE EVALUATIONS
GENETIC ENGINEERING
PLASMIDS
RECOMBINANT DNA
ZYMOMONAS MOBILIS
ALCOHOLS
ALDEHYDES
BACTERIA
BIOCONVERSION
CARBOHYDRATES
CELL CONSTITUENTS
DISACCHARIDES
DNA
HEXOSES
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
NUCLEIC ACIDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
SACCHARIDES
SYNTHESIS
090900* - Biomass Fuels- Processing- (1990-)