Investigation of the mechanism of phosphonoacetaldehyde hydrolase
The authors are presently studying enzymes which catalyze the formation and cleavage of carbon phosphorous bonds. In 1970 LaNauze et. al. reported the isolation of one enzyme of interest - phosphonoacetaldehyde hydrolase from a mutant of Bacillus cereus. This enzyme catalyzes the hydrolysis of phosphonoaldehyde to acetaldehyde and inorganic phosphate. They have isolated phosphonatase from wild type B. cereus (grown on 2-aminoethylphosphonate as the P/sub i/ source) and have used /sup 1/H-NMR and /sup 31/P-NMR techniques to determine the products of the enzyme reaction as phosphate and acetaldehyde. The mechanism of the enzyme could involve the formation of a Schiff base between phosphonoacetaldehyde and lysine or it might only require Mg/sup + +/, an essential cofactor for activity. To distinguish between these possibilities they have begun to look at the Schiff base formation in more detail. NaBH/sub 4/ was found to inactivate the enzyme in the presence of substrate but not in its absence. This is consistent with results obtained for the enzyme isolated from the mutant bacteria. In addition treatment of the wild type enzyme with tritiated NaBH/sub 4/ resulted in significant incorporation of radiolabel into the protein as compared to the control. These results tentatively suggest that hydrolysis proceeds via a covalent imine intermediate.
- Research Organization:
- Univ. of Maryland, College Park
- OSTI ID:
- 5097422
- Report Number(s):
- CONF-8606151-; TRN: 86-034813
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Investigation into the mechanism of the Bacillus cereus phosphonoacetaldehyde hydrolase enzyme
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETALDEHYDE
HYDROLYSIS
HYDROLASES
ENZYME ACTIVITY
PHOSPHATES
BACILLUS
COVALENCE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PHOSPHORUS 31
SCHIFF BASES
ALDEHYDES
BACTERIA
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
IMINES
ISOTOPES
LIGHT NUCLEI
LYSIS
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RESONANCE
SOLVOLYSIS
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques
550600 - Medicine