Reduced platelet-mediated and enhanced leukocyte-mediated fibrinolysis in experimentally induced diabetes in rats
Studies of fibrinolytic activity in diabetes mellitus have produced conflicting results. This may be a result of methodologic insensitivity or of variable contributions of the different blood components to whole blood fibrinolysis. To explore these two possibilities, the authors used a sensitive solid-phase radiometric assay to examine the fibrinolytic activity of whole blood, platelet-rich plasma, leukocytes, and platelet- and leukocyte-poor plasma prepared from control rats and rats with streptozocin-induced diabetes at various times after induction of diabetes. Fibrinolytic activity of whole blood from diabetic rats after 7 days was significantly reduced, and remained reduced after longer durations of diabetes up to 28 days. Platelet-rich plasma from diabetic rats had decreased fibrinolytic activity, which followed the same time course of changes as in whole blood. The platelet contribution to whole blood fibrinolysis was further reduced in vivo after 14 days of diabetes by a reduced whole blood platelet count. In contrast, fibrinolytic activity of leukocytes from diabetic rats became enhanced after 7 days of diabetes. After 49 days of diabetes, the whole blood leukocyte count was reduced, and in vivo would offset the enhanced activity. Plasma fibrinolytic activity was small compared with that of whole blood and was unaltered in diabetic rats. The authors conclude that altered platelet function contributes to decreased fibrinolytic activity of whole blood in diabetic rats, and that this may be partially offset by enhanced leukocyte-mediated fibrinolysis.
- Research Organization:
- Veterans Administration Medical Center, Charleston, SC
- OSTI ID:
- 5019904
- Journal Information:
- J. Lab. Clin. Med.; (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBRINOLYSIS
RADIOASSAY
IODINE ISOTOPES
TRACER TECHNIQUES
BLOOD
BLOOD PLASMA
BLOOD PLATELETS
DIABETES MELLITUS
FIBRIN
GLUCOSE
LEUKOCYTES
RATS
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CHEMICAL REACTIONS
COAGULANTS
DECOMPOSITION
DISEASES
DRUGS
ENDOCRINE DISEASES
HEMATOLOGIC AGENTS
HEMOSTATICS
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
METABOLIC DISEASES
MONOSACCHARIDES
ORGANIC COMPOUNDS
PROTEINS
PROTEOLYSIS
RODENTS
SACCHARIDES
SCLEROPROTEINS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics