Immunological identification of human T cells intracranially and tracing of neuronal projections by magnetic resonance imaging
This report describes the preparation and utilization of paramagnetically labelled proteins to trace neural projections in vivo, and to distinguish between human T cells and bovine T cells implanted into canine brain. The proteins are covalently coupled to the chelator (DTPA), then labelled with gadolinium and visualized in vivo by magnetic resonance imaging (MRI) techniques. Gadolinium labelled horseradish peroxidase (HRP) was injected into the auditory cortex of adult cats (1-7 ..mu..1 containing 50 ..mu..g HRP per ..mu..1) and 48-72 hours later the brain was imaged by MRI. The HRP was labelled with an average of 20 DTPA per HRP. MRI unambiguously identified the HRP injection sites and the sites of neural projections in the medical geniculate body (MGB). MGB localization of HRP-Gd was confirmed histologically demonstrating that MRI can distinguish between paramagnetically labelled protein and local environment effects in the brain (i.e. gray vs white matter). Two monoclonal antibodies against human T cells were labelled with gadolinium. The distinguished by MRI, human from bovine T cells implanted into canine brains (each implant contained 10 million cells in 40 ..mu..1). The T1 weighted and calculated images readily identified the human T cells as a lesion of <4 mm while the bovine T cells did not yield a significant MRI signal. The ratio of DTPA to protein during the coupling procedure, affects the formation of protein aggregates by crosslinking.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 5018274
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
IMPLANTS
PROTEINS
LABELLING
BRAIN
CATS
CATTLE
COVALENCE
CROSS-LINKING
DTPA
GADOLINIUM ISOTOPES
MAN
MONOCLONAL ANTIBODIES
NERVES
NUCLEAR MAGNETIC RESONANCE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHELATING AGENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DOMESTIC ANIMALS
DRUGS
ISOTOPES
LEUKOCYTES
MAGNETIC RESONANCE
MAMMALS
MATERIALS
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PRIMATES
RADIOPROTECTIVE SUBSTANCES
RARE EARTH ISOTOPES
RESONANCE
RUMINANTS
SOMATIC CELLS
VERTEBRATES
550600* - Medicine
550201 - Biochemistry- Tracer Techniques