Total lymphoid irradiation in kidney and liver transplantation in the baboon: prolonged graft survival and alterations in T cell subsets with low cumulative dose regimens
Prolonged kidney and liver allograft survival was produced in baboons by low cumulative doses (500 to 1200 rad) of total lymphoid irradiation (TLI). Continuing normal graft function for more than 1 year after transplantation was seen in 10 animals in this series. The longest survivor (> 4 years) rejected a third party kidney allograft in typically acute fashion 1 yr after the original transplantation. The dose-response effect was parabolic, with a cumulative dose of 800 rad given as twice weekly fractions of 100 rad each being the most effective. With smaller cumulative doses (500 or 600 rad) results were improved if TLI was administered as a large number of smaller fractions. These regimens are free of the radiation related mortality observed in earlier studies with larger cumulative doses. Alterations in T cell subpopulations were studied in these baboons with the use of the anti-human monoclonal antibodies OKT11 (anti-total T cells), OKT4 (anti-T helper cells; Th), and OKT8 (anti-T suppressor/cytotoxic cells, Tsc), which cross-react with baboon lymphocytes. After completion of TLI but before transplantation, the relative percentage of Tsc cells fell and the percentage of Th and the Th/Tsc ratio rose. Transplantation was followed by an inversion of this ratio due to reciprocal changes in the percentages of Th and Tsc cells. This pattern persisted and increased until 6 mo after transplantation, when it reverted to that seen in untreated control animals.
- Research Organization:
- Univ. of the Witwatersrand, Johannesburg, South Africa
- OSTI ID:
- 5003607
- Journal Information:
- J. Immunol.; (United States), Vol. 132:2
- Country of Publication:
- United States
- Language:
- English
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RADIATION DOSES
IMMUNOSUPPRESSION
RADIOINDUCTION
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
IRRADIATION PROCEDURES
KIDNEYS
LIVER
MONOCLONAL ANTIBODIES
THYMUS CELLS
TRANSPLANTS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
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BODY FLUIDS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DOSES
GLANDS
LEUKOCYTES
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560152* - Radiation Effects on Animals- Animals