Alternative splicing of the FMR1 gene in human fetal brain neurons
- Chinese Academy of Medical Sciences, Beijing (China); and others
The alternative splicing expression of the FMR1 gene was reported in several human and mouse tissues. Five regions of FMR1 gene can be alternatively spliced, but the combination of them has not been investigated fully. We reported here the analysis of alternative splicing pattern of the FMR1 gene in cultured fetal human neurons, using a RT-PCR and cloning strategy. Eleven splicing types were cloned and different isoforms were not equally represented. The dominant isoform represents nearly 40%, and the other isoforms were relatively rare. One isoform has a different carboxyl-terminus. Most of the alternative spliced regions appear hydrophilic; thus, they may locate on the surface of the FMR1 protein. 16 refs., 2 figs.
- OSTI ID:
- 478874
- Journal Information:
- American Journal of Medical Genetics, Vol. 64, Issue 2; Other Information: PBD: 9 Aug 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
HUMAN X CHROMOSOME
CHROMOSOMAL ABERRATIONS
GENETIC MAPPING
GENES
SPLICING
GENE REGULATION
DNA-CLONING
TRANSCRIPTION
DNA SEQUENCING
FETUSES
ONTOGENESIS
HEREDITARY DISEASES
MENTAL DISORDERS
PROTEINS
NERVE CELLS
POLYMERASE CHAIN REACTION
HIPPOCAMPUS
ELECTROPHORESIS