Use of microbial encapsulation/immobilization for biodegradation of PAHs
- Sybron Chemicals, Inc., Salem, VA (United States). Biochemical and Environmental Services
- SBP Technologies, Inc., Gulf Breeze, FL (United States)
- Naval Research Lab., Edgewater, MD (United States)
- Environmental Protection Agency, Gulf Breeze, FL (United States)
Bioaugmentation as a strategy in bioremediation has great potential but has had little success to support its use. Problems have arisen because of a general inability to support the growth and/or activity of the introduced organism in the environment because of competition factors, poor survival of the inoculum, and grazing by protozoa. A specialized technique that has been used to overcome these problems is cell immobilization or encapsulation, in which the inoculant can be placed in environmental media in a way that reduces competition from the indigenous microflora and allows expression of the specific introduced metabolic function. Packaging of specific bacterial or fungal cells in a porous polymeric material potentially improves storage of inocula, and enhances the capability of directly introducing viable and active cells into environmental material at some future time without the need to regrow the cells. The authors have been experimenting with encapsulation;immobilization procedures for use in the bioremediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil. In this paper, the authors demonstrate the potential usefulness of polyurethane foam and vermiculite for this purpose and show that optimal PAH degradation can be maintained with immobilized cells.
- OSTI ID:
- 474252
- Report Number(s):
- CONF-950483-; ISBN 1-57477-004-7; TRN: IM9723%%142
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioaugmentation for site remediation; Hinchee, R.E.; Fredrickson, J.; Alleman, B.C. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; PB: 272 p.; Bioremediation, Volume 3(3)
- Country of Publication:
- United States
- Language:
- English
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