Expression of the human muscarinic receptor gene m2 in Dictyostelium discoideum
- Johann Wolfgang Goethe Universitaet, Frankfurt (Germany)
We have expressed a functional human muscarinic M2 receptor, under the control of the homologous discoidin I{gamma} promoter, in the cellular slime mold Dictyostelium discoideum. The use of a contact site A leader peptide ensured insertion of the newly synthesized receptor protein into the plasma membrane. Due to the characteristics of the discoidin I{gamma} promoter, the M2 receptor is expressed during late growth and early development. The heterologously expressed M2 receptors show binding characteristics similar to authentic receptors. Membranes as well as whole cells can be used in ligand binding assays. 36 refs., 4 figs.
- OSTI ID:
- 457527
- Journal Information:
- Bio/Technology, Vol. 13, Issue 11; Other Information: PBD: Nov 1995
- Country of Publication:
- United States
- Language:
- English
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