Fibroblast radiosensitivity in vitro and lung fibrosis in vivo: Comparison between a fibrosis-prone and fibrosis-resistant mouse strain
- Univ. of Texas M.D. Anderson Cancer Center, Houston, TX (United States)
Radiation-induced pneumonitis and fibrosis in the lung after treatment to the thoracic cavity for malignant disease currently limit the maximum tolerated dose to that region. It has been suggested that heterogeneity in susceptibility to radiation-induced fibrosis exists in the population, implying that the lung tolerance dose is defined by a sensitive subset of the patient population. Studies of radiotherapy patients have indicated that the survival at 2 Gy (SF2) of cultured skin fibroblasts correlates with the incidence and severity of postirradiation damage in a number of tissues, suggesting that this assay may be a useful predictor of late tissue effects. The goal of the studies presented here was to determine if the radiosensitivity of fibroblasts in vitro isolated form mouse lungs was correlated with the severity of radiation induced fibrosis in the lungs of two in red strains of mice previously shown to differ markedly in their susceptibility to radiation-induced lung fibrosis: the C3Hf/Kam strain, classified as fibrosis-resistant, and the C57BL/6J strain, classified as fibrosisprone. Quantitative measurements of lung fibrosis after irradiation were compared to SF 2 values for fibroblasts of skin and lung cultured form each strain. Lung fibrosis was quantified, using computerized image analysis, as the percentage of fibrosis on Masson`s Trichrome-stained lung sections from both strains after single doses of radiation to the thorax. For the measurements of SF2, fibroblasts plated at the second passage and grown to confluence were given single doses of radiation ranging from 0 to 6 Gy. 30 refs., 4 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 381034
- Journal Information:
- Radiation Research, Vol. 146, Issue 1; Other Information: PBD: Jul 1996
- Country of Publication:
- United States
- Language:
- English
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