Clone ordering by simulated annealing: Application to the STS-content map of chromosome 21
- Genethon, Paris (France)
This article presents an application of the simulated annealing algorithm used at Genethon for the STS-content map of the chromosome 21. This algorithm is a part of an integrated system which starts from the PCR gel analysis and produces ordered contigs that can be handled with a graphical user interface. For this project, 250 STSs have been used to screen a 14 genome equivalent YAC library. The result is a map of all of the long arm of chromosome 21 (21q). This map contains 210 STSs and 770 YACs and covers a 45 Megabase region with an average resolution of 1 STS/220 kb. The order obtained by simulated annealing is consistent both with genetic data and with other methods of physical mapping (RCRF, alu-PCR). This map will be a powerful tool for gene analysis, especially in the study of Down syndrome and Alzheimer disease.
- OSTI ID:
- 37531
- Report Number(s):
- CONF-9206273-; ISBN 981-02-1157-0; TRN: IM9519%%455
- Resource Relation:
- Conference: 2. international conference on bioinformatics, supercomputing, and complex genome analysis, St. Petersburg, FL (United States), 4-7 Jun 1992; Other Information: PBD: 1993; Related Information: Is Part Of The second international conference on bioinformatics, supercomputing and complex genome analysis; Lim, H.A. [ed.] [Florida State Univ., Tallahassee, FL (United States). Supercomputer Computations Research Inst.]; Fickett, J.W. [ed.] [Los Alamos National Lab., Los Alamos, NM (United States). Center for Human Genome Studies]; Cantor, C.R. [ed.] [Boston Univ., MA (United States). Center for Advanced Research in Biotechnology]; Robbins, R.J. [ed.] [Johns Hopkins Univ., Baltimore, MD (United States). Applied Research Lab.]; PB: 672 p.
- Country of Publication:
- United States
- Language:
- English
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