Evaluation of immunoassay-based field test kits for the detection of petroleum fuel hydrocarbons in soil
- Oak Ridge National Lab., TN (United States)
The objectives of this project are to identify, experimentally evaluate and implement the use of alternative field screening methods that are specific for environmental contaminants of interest and concern to the Department of Energy. Immunochemical techniques are rapidly becoming a significant component in the arsenal of field screening methods. Analytical results obtained by immunoassay have been shown to correlate well with those obtained by traditional laboratory methods. Also, the use of immunoassay-based field screening methods can significantly reduce the cost and time required for environmental assessment. For example, the previous experimental evaluations of immunoassay-based tests for PCBs and mercury showed them to be effective, rapid and economical field screening methods (Methods OS020 and MB100, DOE Methods Compendium) are currently evaluating the effectiveness of immunoassay-based test kits, from a number of sources, for detecting fuel hydrocarbons (primarily BTEX) in soil. The formats of the kits being evaluated vary significantly--from how the test sample is diluted for assay to how the test color reaction is developed and measured. Dilution schemes have been shown to be critical, due to the volatility of the analytes; and can result in false negative results. Kits, with which the color reaction development occurs on a membranous filter, are subject to producing erroneous and erratic results if the test samples and/or test reagents are compromised in such a way as to impede the normal flow rate through the filter. Results of these studies, with respect to the accuracy, reproducibility, sensitivity, working range, cost and sample throughput, will be presented.
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 276394
- Report Number(s):
- CONF-950209-; TRN: 96:017948
- Resource Relation:
- Conference: 4. international symposium on field screening methods for hazardous wastes and toxic chemicals, Las Vegas, NV (United States), 22-24 Feb 1995; Other Information: PBD: 1995; Related Information: Is Part Of Field screening methods for hazardous wastes and toxic chemicals. VIP-47, Volume 2; PB: 701 p.
- Country of Publication:
- United States
- Language:
- English
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