Biological effects of low-level laser irradiation on umbilical cord mesenchymal stem cells

Journal Article · Fri Apr 15 00:00:00 EDT 2016 · AIP Advances

DOI:https://doi.org/10.1063/1.4948442· OSTI ID:22611646

Chen, Hongli; Wang, Hong; Li, Yingxin; Liu, Weichao; Chen, Zhuying ^[1]; Wang, Chao ^[2]

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1. Key Laboratory of Laser Medicine of Tianjin, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192 (China)
2. Biomedical Engineering and Technology College, Tianjin Medical University, Tianjin, 300070 (China)

Low-level laser irradiation (LLLI) can enhance stem cell (SC) activity by increasing migration and proliferation. This study investigated the effects of LLLI on proliferation, enzymatic activity, and growth factor production in human umbilical cord mesenchymal SCs (hUC-MSCs) as well as the underlying mechanisms. hUC-MSCs were assigned to a control group (non-irradiation group) and three LLLI treatment groups (635 nm group, 808 nm group, and 635/808 nm group). Laser power density and energy density of 20 mW/cm{sup 2} and 12 J/cm{sup 2}, respectively, were used for each experiment. The proliferation rate was higher in the 635 nm as compared to the other groups. LLLI at 808 nm did not induce cell proliferation. ROS levels in cells exposed to 635, 808, and 635/808 nm radiation were increased by 52.81%, 26.89%, and 21.15%, respectively, relative to the control group. CAT, tGPx, and SOD activity was increased. LLLI at 808 nm increased the levels of IL-1, IL-6, and NFκB but not VEGF. LLLI improved hUC-MSCs function and increased antioxidant activity. Dual-wavelength LLLI had more potent effects on hUC-MSCs than single-wavelength treatment. LLLI has potential applications in the preconditioning of hUC-MSCs in vitro prior to transplantation, which could improve the regenerative capacity of cells.

• OSTI ID: 22611646
• Journal Information: AIP Advances, Vol. 6, Issue 4; Other Information: (c) 2016 Author(s); Country of input: International Atomic Energy Agency (IAEA); ISSN 2158-3226
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
ANTIOXIDANTS
BIOLOGICAL EFFECTS
CAPACITY
CELL PROLIFERATION
COMPARATIVE EVALUATIONS
CONTROL
ENERGY DENSITY
GROWTH FACTORS
IRRADIATION
LASER RADIATION
POWER DENSITY
STEM CELLS
SUPEROXIDE DISMUTASE
WAVELENGTHS