The characterization of exosome from blood plasma of patients with colorectal cancer

Journal Article · Tue Aug 02 00:00:00 EDT 2016 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4960289· OSTI ID:22608294

Yunusova, N. V., E-mail: [e-mail] ^[1]; Siberian State Medical University, Moskovsky Trakt 2, Tomsk, 634050 ^[2]; Tamkovich, S. N., E-mail: [e-mail] ^[3]; Novosibirsk State University, Pirogov Street 2, Novosibirsk, 630090 ^[2]; Stakheeva, M. N., E-mail: [e-mail]; Afanas’ev, S. G., E-mail: [e-mail]; Kondakova, I. V., E-mail: [e-mail] ^[1]; Frolova, A. Y., E-mail: [e-mail] ^[4]

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1. Tomsk Cancer Research Institute, Kooperativny Street 5, Tomsk, 634009 (Russian Federation)
2. (Russian Federation)
3. Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk, 630090 (Russian Federation)
4. Siberian State Medical University, Moskovsky Trakt 2, Tomsk, 634050 (Russian Federation)

Exosomes are extracellular membrane structures involved in many physiological and pathological processes including cancerogenesis and metastasis. The clarification of the criteria for exosome isolating and identifying is the purpose of this study. Exosome samples from the plasma of patients with colorectal cancer and healthy donors were examined using transmission electron microscopy and flow cytometry in accordance with the minimum requirements of “International Society for Extracellular Vesicles”. The choice of the method for isolation of exosomes from the blood plasma by ultrafiltration and ultracentrifugation allowed obtaining highly purified samples of exosomes, in which all the structural components were clearly seen. The results obtained with flow cytometry suggest that exosomes of blood plasma from patients with colorectal cancer can be produced by epithelial cells. Moreover, cells produce different types of exosomes, which correspond to different mechanisms in sorting macromolecules in the membrane of multivesicular bodies. Determination of significant differences in the expression of specific exosomal proteins from colorectal cancer patients compared to healthy donors suggests a high diagnostic potential significance of circulating exosomes.

• OSTI ID: 22608294
• Journal Information: AIP Conference Proceedings, Vol. 1760, Issue 1; Conference: PC'16: International conference on physics of cancer: Interdisciplinary problems and clinical applications 2016, Tomsk (Russian Federation), 22-25 Mar 2016; Other Information: (c) 2016 Author(s); Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD PLASMA
COMPARATIVE EVALUATIONS
ELECTRONS
MEMBRANES
METASTASES
NEOPLASMS
PATIENTS
PLASMA
TRANSMISSION
TRANSMISSION ELECTRON MICROSCOPY
ULTRACENTRIFUGATION
ULTRAFILTRATION