Repeatability and reproducibility of intracellular molar concentration assessed by synchrotron-based x-ray fluorescence microscopy

Journal Article · Thu Jan 28 00:00:00 EST 2016 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4937526· OSTI ID:22494396

Gianoncelli, A. ^[1]; Cappadone, C.; Farruggia, G.; Sargenti, A.; Procopio, A. ^[2]; Fratini, M. ^[3]; Notargiacomo, A. ^[4]; Lombardo, M. ^[5]; Lagomarsino, S. ^[6]; Iotti, S. ^[2]

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1. Elettra - Sincrotrone Trieste, 34149 Basovizza, Trieste (Italy)
2. Department of Pharmacy and Biotechnology, University of Bologna, Bologna 40127 (Italy)
3. Museo Storico della Fisica e Centro Studi e Ricerche Enrico Fermi, Piazza del Viminale 1, 00184 Roma Italy (Italy)
4. Institute for Photonics and Nanotechnology, Consiglio Nazionale delle Richerche, 00156 Rome (Italy)
5. Department of Chemistry “G. Ciamician”, University of Bologna, Bologna 40126 (Italy)
6. Institute of Chemical-Physical Processes, Sapienza University of Rome, 00185 Rome (Italy)

Elemental analysis of biological sample can give information about content and distribution of elements essential for human life or trace elements whose absence is the cause of abnormal biological function or development. However, biological systems contain an ensemble of cells with heterogeneous chemistry and elemental content; therefore, accurate characterization of samples with high cellular heterogeneity may only be achieved by analyzing single cells. Powerful methods in molecular biology are abundant, among them X-Ray microscopy based on synchrotron light source has gaining increasing attention thanks to its extremely sensitivity. However, reproducibility and repeatability of these measurements is one of the major obstacles in achieving a statistical significance in single cells population analysis. In this study, we compared the elemental content of human colon adenocarcinoma cells obtained by three distinct accesses to synchrotron radiation light.

• OSTI ID: 22494396
• Journal Information: AIP Conference Proceedings, Vol. 1696, Issue 1; Conference: XRM 2014: 12. international conference on X-ray microscopy, Melbourne (Australia), 26-31 Oct 2014; Other Information: (c) 2016 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
AUGMENTATION
BIOLOGICAL FUNCTIONS
CARCINOMAS
CHEMISTRY
COMPARATIVE EVALUATIONS
CONCENTRATION RATIO
FLUORESCENCE
HUMAN POPULATIONS
LARGE INTESTINE
LIGHT SOURCES
MICROSCOPY
MOLECULAR BIOLOGY
SENSITIVITY
SYNCHROTRON RADIATION
SYNCHROTRONS
TRACE AMOUNTS
X RADIATION