skip to main content

SciTech ConnectSciTech Connect

Title: GATA-1 directly regulates Nanog in mouse embryonic stem cells

Nanog safeguards pluripotency in mouse embryonic stem cells (mESCs). Insight into the regulation of Nanog is important for a better understanding of the molecular mechanisms that control pluripotency of mESCs. In a silico analysis, we identify four GATA-1 putative binding sites in Nanog proximal promoter. The Nanog promoter activity can be significantly repressed by ectopic expression of GATA-1 evidenced by a promoter reporter assay. Mutation studies reveal that one of the four putative binding sites counts for GATA-1 repressing Nanog promoter activity. Direct binding of GATA-1 on Nanog proximal promoter is confirmed by electrophoretic mobility shift assay and chromatin immunoprecipitation. Our data provide new insights into the expanded regulatory circuitry that coordinates Nanog expression. - Highlights: • The Nanog proximal promoter conceives functional element for GATA-1. • GATA-1 occupies the Nanog proximal promoter in vitro and in vivo. • GATA-1 transcriptionally suppresses Nanog.
Authors:
;  [1] ;  [2] ;  [3] ;  [1] ;  [2] ;  [4] ;  [2] ;  [4] ;  [2]
  1. College of Life Sciences, Northwest A&F University, Yangling 712100 (China)
  2. (China)
  3. School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027 (China)
  4. College of Veterinary Medicine, Northwest A&F University, Yangling 712100 (China)
Publication Date:
OSTI Identifier:
22458564
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 465; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CHROMATIN; COORDINATES; ELECTROPHORESIS; IN VITRO; IN VIVO; MICE; MOBILITY; MUTATIONS; PROMOTERS; SAFEGUARDS; STEM CELLS