skip to main content

SciTech ConnectSciTech Connect

Title: Autographa californica multiple nucleopolyhedrovirus PK-1 is essential for nucleocapsid assembly

PK-1 (Ac10) is a baculovirus-encoded serine/threonine kinase and its function is unclear. Our results showed that a pk-1 knockout AcMNPV failed to produce infectious progeny, while the pk-1 repair virus could rescue this defect. qPCR analysis demonstrated that pk-1 deletion did not affect viral DNA replication. Analysis of the repaired recombinants with truncated pk-1 mutants demonstrated that the catalytic domain of protein kinases of PK-1 was essential to viral infectivity. Moreover, those PK-1 mutants that could rescue the infectious BV production defect exhibited kinase activity in vitro. Therefore, it is suggested that the kinase activity of PK-1 is essential in regulating viral propagation. Electron microscopy revealed that pk-1 deletion affected the formation of normal nucleocapsids. Masses of electron-lucent tubular structures were present in cell transfected with pk-1 knockout bacmid. Therefore, PK-1 appears to phosphorylate some viral or cellular proteins that are essential for DNA packaging to regulate nucleocapsid assembly. - Highlights: • A pk-1 knockout AcMNPV failed to produce infectious progeny. • The pk-1 deletion did not affect viral DNA replication. • The catalytic domain of protein kinases (PKc) of PK-1 was essential to viral infectivity. • The kinase activity of PK-1 is essential in regulating viral propagation. • PK-1more » appears to phosphorylate some viral proteins that are essential for DNA packaging to regulate nucleocapsid assembly.« less
Authors:
 [1] ; ; ; ; ; ;  [1] ; ;  [2]
  1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009 (China)
  2. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071 (China)
Publication Date:
OSTI Identifier:
22436640
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 443; Journal Issue: 2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DNA; DNA REPLICATION; ELECTRON MICROSCOPY; IN VITRO; INFECTIVITY; MUTANTS; PACKAGING; PHOSPHOTRANSFERASES; PROGENY; REPAIR; SERINE; THREONINE; VIRUSES