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Title: Inhibition of N-acetylglucosaminyltransferase V enhances sensitivity of radiotherapy in human prostate cancer

Highlights: • We first evaluated the effect of GnT-V on radiation sensitivity of prostate cancer. • Higher level of GnT-V was detected more frequently in the PCa advanced tumors. • Attenuation of GnT-V inhibited cell proliferation, migration and increased apoptosis. • Knockdown of GnT-V could decrease radiation-induced G2/M arrest and NF-κB activity. • Inhibition of GnT-V may be involved in increasing radiation sensitivity of PCa cells. - Abstract: The purpose of this study was to investigate the relationship between N-acetylglucosaminyltransferase V (GnT-V) and radiation sensitivity of prostate cancer (PCa) cells both in vitro and in vivo. Firstly, the GnT-V expression was studied in 84 cases of PCa tissues, in which higher level of GnT-V was detected more frequently in the advanced tumors. Secondly, the GnT-V stably suppressed cell lines PCa/1079 (Lncap/1079 and PC3/1079) were constructed from PCa cell lines (Lncap and PC3) in vitro. Attenuation of GnT-V inhibited cell proliferation, migration and increased apoptosis, which resulted in enhanced radiation sensitivity of PCa cells. The underlying mechanism may be relevant to the increasing ratio of Bax/Bcl-2, the blocking transcription of NF-κB and the reduction of cell cycle G2-M arrest. Finally, in in vivo study, compared with control groups, the irradiated PCamore » xenograft nude mice of PCa/1079 indicated to reduce tumor-growth rate and enhance survival time. Summary, our studies showed that inhibition of GnT-V probably improved PCa cells’ radiation sensitivity.« less
Authors:
;  [1] ;  [2] ; ; ; ;  [1] ;  [1]
  1. Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282 (China)
  2. Department of Oncology, First People’s Hospital of Jiujiang City, Jiujiang 332000 (China)
Publication Date:
OSTI Identifier:
22416723
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 451; Journal Issue: 3; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; APOPTOSIS; BORON CHLORIDES; CELL CYCLE; CELL PROLIFERATION; HUMAN POPULATIONS; IN VITRO; IN VIVO; INHIBITION; IRRADIATION; MICE; NEOPLASMS; PROSTATE; RADIOSENSITIVITY; RADIOTHERAPY; SURVIVAL TIME; TRANSCRIPTION