skip to main content

Title: Biological activities of Schottenol and Spinasterol, two natural phytosterols present in argan oil and in cactus pear seed oil, on murine miroglial BV2 cells

Highlights: • Sterol composition in argan oil and in cactus seed oil. • Chemical synthesis of two sterols: Schottenol and Spinasterol. • Sterols from argan oil or from cactus seed oil show no toxicity on BV2 cells. • Schottenol and Spinasterol modulate the activation and the expression of two nuclear receptors, LXRα and LXRβ. - Abstract: The objective of this study was to evaluate the biological activities of the major phytosterols present in argan oil (AO) and in cactus seed oil (CSO) in BV2 microglial cells. Accordingly, we first determined the sterol composition of AO and CSO, showing the presence of Schottenol and Spinasterol as major sterols in AO. While in CSO, in addition to these two sterols, we found mainly another sterol, the Sitosterol. The chemical synthesis of Schottenol and Spinasterol was performed. Our results showed that these two phytosterols, as well as sterol extracts from AO or CSO, are not toxic to microglial BV2 cells. However, treatments by these phytosterols impact the mitochondrial membrane potential. Furthermore, both Schottenol and Spinasterol can modulate the gene expression of two nuclear receptors, liver X receptor (LXR)-α and LXRβ, their target genes ABCA1 and ABCG1. Nonetheless, only Schottenol exhibited a differential activationmore » vis-à-vis the nuclear receptor LXRβ. Thus Schottenol and Spinasterol can be considered as new LXR agonists, which may play protective roles by the modulation of cholesterol metabolism.« less
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [1] ;  [1] ;  [2] ; ;  [1] ;  [5] ;  [6] ;  [1] ;  [7] ;  [4] ;
  1. Université de Bourgogne, Laboratoire Bio-PeroxIL, EA7270, Dijon F-21000 (France)
  2. (Morocco)
  3. LCPMC-A2, ICPM, Department of Chemistry, Université de Lorraine, Metz (France)
  4. CRINSERM 866, Dijon (France)
  5. INSERM and HMNO, CBP, CHRU Lille, 59037 Lille (France)
  6. Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan I, BP 577, 26000 Settat (Morocco)
  7. Laboratoire de recherche sur les Lipoprotéines et l’Athérosclérose, Faculté des Sciences Ben M’sik, Avenue Cdt Driss El Harti BP. 7955, Université Hassan II-Mohammedia-Casablanca (Morocco)
Publication Date:
OSTI Identifier:
22416372
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 446; Journal Issue: 3; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CACTI; CELL MEMBRANES; CHOLESTEROL; GENES; LIVER; METABOLISM; MITOCHONDRIA; OILS; RECEPTORS; SITOSTEROL; SYNTHESIS; TOXICITY