skip to main content

Title: Single-walled carbon nanotubes based chemiresistive genosensor for label-free detection of human rheumatic heart disease

A specific and ultrasensitive, label free single-walled carbon nanotubes (SWNTs) based chemiresistive genosensor was fabricated for the early detection of Streptococcus pyogenes infection in human causing rheumatic heart disease. The mga gene of S. pyogenes specific 24 mer ssDNA probe was covalently immobilized on SWNT through a molecular bilinker, 1-pyrenemethylamine, using carbodiimide coupling reaction. The sensor was characterized by the current-voltage (I-V) characteristic curve and scanning electron microscopy. The sensing performance of the sensor was studied with respect to changes in conductance in SWNT channel based on hybridization of the target S. pyogenes single stranded genomic DNA (ssG-DNA) to its complementary 24 mer ssDNA probe. The sensor shows negligible response to non-complementary Staphylococcus aureus ssG-DNA, confirming the specificity of the sensor only with S. pyogenes. The genosensor exhibited a linear response to S. pyogenes G-DNA from 1 to1000 ng ml{sup −1} with a limit of detection of 0.16 ng ml{sup −1}.
Authors:
;  [1] ;  [2] ;  [3] ;  [4] ;  [5]
  1. CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007 (India)
  2. (AcSIR), New Delhi (India)
  3. National Centre for Disease Control, Sham Nath Marg, Delhi 110054 (India)
  4. Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521 (United States)
  5. CSIR-National Physical Laboratory, Dr. K. S. Krishnan Road, New Delhi 110012 (India)
Publication Date:
OSTI Identifier:
22392083
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Physics Letters; Journal Volume: 105; Journal Issue: 21; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CARBON NANOTUBES; CARDIOVASCULAR DISEASES; DNA; ELECTRIC CONDUCTIVITY; GENES; HUMAN POPULATIONS; HYBRIDIZATION; PERFORMANCE; SCANNING ELECTRON MICROSCOPY; SENSORS; STAPHYLOCOCCUS; STREPTOCOCCUS