Crystallization of Ranasmurfin, a blue-coloured protein from Polypedates leucomystax

Journal Article · Wed Nov 01 00:00:00 EST 2006 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S1744309106040036· OSTI ID:22356396

McMahon, Stephen A. ^[1]; Walsh, Martin A. ^[2]; Ching, Rosalind Tan Yan ^[3]; Carter, Lester G.; Dorward, Mark; Johnson, Kenneth A.; Liu, Huanting; Oke, Muse ^[1]; Bloch, Carlos Jr ^[4]; Kennedy, Malcolm W. ^[5]; Latiff, Aishah A. ^[6]; Cooper, Alan ^[3]; Taylor, Garry L.; White, Malcolm F.

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1. Centre for Biomolecular Science and The Scottish Structural Proteomics Facility, The University of St Andrews Fife KY16 9RH,Scotland (United Kingdom)
2. Medical Research Council France, European Synchrotron Radiation Facility, BP 220, F-38043 Grenoble CEDEX (France)
3. WestCHEM, Department of Chemistry, University of Glasgow, Glasgow G12 8QQ,Scotland (United Kingdom)
4. Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Asa Norte, Brasilia 70910-900 (Brazil)
5. Division of Environmental and Evolutionary Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ,Scotland (United Kingdom)
6. Doping Control Centre Penang, Universiti Sains Malaysia, 11800 Minden, Penang (Malaysia)

A novel blue protein from frog nests has been crystallized. Ranasmurfin, a previously uncharacterized ∼13 kDa blue protein found in the nests of the frog Polypedates leucomystax, has been purified and crystallized. The crystals are an intense blue colour and diffract to 1.51 Å with P2{sub 1} symmetry and unit-cell parameters a = 40.9, b = 59.9, c = 45.0 Å, β = 93.3°. Self-rotation function analysis indicates the presence of a dimer in the asymmetric unit. Biochemical data suggest that the blue colour of the protein is related to dimer formation. Sequence data for the protein are incomplete, but thus far have identified no model for molecular replacement. A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution.

• OSTI ID: 22356396
• Journal Information: Acta Crystallographica. Section F, Vol. 62, Issue Pt 11; Other Information: PMCID: PMC2225219; PMID: 17077494; PUBLISHER-ID: hc5016; OAI: oai:pubmedcentral.nih.gov:2225219; Copyright (c) International Union of Crystallography 2006; This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html.; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
DIMERS
FLUORESCENCE
MATHEMATICAL SOLUTIONS
PEAKS
PROTEINS
ROTATION
SOLUTIONS
SYMMETRY
ZINC