Crystallization and preliminary X-ray analysis of the Man(α1-2)Man-specific lectin from Bowringia mildbraedii in complex with its carbohydrate ligand

Journal Article · Sat Oct 01 00:00:00 EDT 2005 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/S174430910502854X· OSTI ID:22356216

Garcia-Pino, Abel; Loris, Remy; Wyns, Lode; Buts, Lieven ^[1]

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1. Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel and Department of Molecular and Cellular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Pleinlaan 2, B-1050 Brussel (Belgium)

The lectin from the Nigerian legume B. mildbraedii was crystallized in complex with Man(α1-2)Man and data were collected to a resolution of 1.90 Å using synchrotron radiation. The lectin from Bowringia mildbraedii seeds crystallizes in the presence of the disaccharide Man(α1-2)Man. The best crystals grow at 293 K within four weeks after a pre-incubation at 277 K to induce nucleation. A complete data set was collected to a resolution of 1.90 Å using synchrotron radiation. The crystals belong to space group I222, with unit-cell parameters a = 66.06, b = 86.35, c = 91.76 Å, and contain one lectin monomer in the asymmetric unit.

• OSTI ID: 22356216
• Journal Information: Acta Crystallographica. Section F, Vol. 61, Issue Pt 10; Other Information: PMCID: PMC1991310; PMID: 16511199; PUBLISHER-ID: ll5037; OAI: oai:pubmedcentral.nih.gov:1991310; Copyright (c) International Union of Crystallography 2005; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
• Country of Publication: United Kingdom
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
CRYSTALS
LIGANDS
MONOMERS
NUCLEATION
RESOLUTION
SPACE GROUPS
SYNCHROTRON RADIATION