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Title: Improvements in the order, isotropy and electron density of glypican-1 crystals by controlled dehydration

The anisotropy of crystals of glypican-1 was significantly reduced by controlled dehydration using the HC1 device, allowing the building of previously disordered parts of the structure. The use of controlled dehydration for improvement of protein crystal diffraction quality is increasing in popularity, although there are still relatively few documented examples of success. A study has been carried out to establish whether controlled dehydration could be used to improve the anisotropy of crystals of the core protein of the human proteoglycan glypican-1. Crystals were subjected to controlled dehydration using the HC1 device. The optimal protocol for dehydration was developed by careful investigation of the following parameters: dehydration rate, final relative humidity and total incubation time T{sub inc}. Of these, the most important was shown to be T{sub inc}. After dehydration using the optimal protocol the crystals showed significantly reduced anisotropy and improved electron density, allowing the building of previously disordered parts of the structure.
Authors:
 [1] ;  [2] ;  [3] ;  [1] ;  [4] ;  [3] ;  [1]
  1. Lund University, Box 124, 221 00 Lund (Sweden)
  2. (Egypt)
  3. Lund University, Biomedical Center A13, 221 84 Lund (Sweden)
  4. (Sweden)
Publication Date:
OSTI Identifier:
22347806
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 69; Journal Issue: Pt 12; Other Information: PMCID: PMC3852657; PMID: 24311593; PUBLISHER-ID: rr5050; OAI: oai:pubmedcentral.nih.gov:3852657; Copyright (c) Awad et al. 2013; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ANISOTROPY; AUGMENTATION; CRYSTALS; DEHYDRATION; DENSITY; DIFFRACTION; ELECTRON DENSITY; ELECTRONS; EQUIPMENT; HUMIDITY; ISOTROPY; OPTIMIZATION; PROTEINS