Exploiting fast detectors to enter a new dimension in room-temperature crystallography
Journal Article
·
· Acta Crystallographica. Section D: Biological Crystallography
- Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE (United Kingdom)
- Dectris Ltd, Neuenhofer Strasse 107, 5400 Baden (Switzerland)
- University of Oxford, The Henry Wellcome Building for Genomic Medicine, Roosevelt Drive, Oxford OX3 7BN (United Kingdom)
A departure from a linear or an exponential decay in the diffracting power of macromolecular crystals is observed and accounted for through consideration of a multi-state sequential model. A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerable intensity decay is reached. A simple model accounting for the form of the intensity decay is reintroduced and is applied for the first time to high frame-rate room-temperature data collection.
- OSTI ID:
- 22347797
- Journal Information:
- Acta Crystallographica. Section D: Biological Crystallography, Vol. 70, Issue Pt 5; Other Information: PMCID: PMC4014120; PMID: 24816094; PUBLISHER-ID: wa5063; OAI: oai:pubmedcentral.nih.gov:4014120; Copyright (c) Owen et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0907-4449
- Country of Publication:
- Denmark
- Language:
- English
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