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Title: Exploiting fast detectors to enter a new dimension in room-temperature crystallography

A departure from a linear or an exponential decay in the diffracting power of macromolecular crystals is observed and accounted for through consideration of a multi-state sequential model. A departure from a linear or an exponential intensity decay in the diffracting power of protein crystals as a function of absorbed dose is reported. The observation of a lag phase raises the possibility of collecting significantly more data from crystals held at room temperature before an intolerable intensity decay is reached. A simple model accounting for the form of the intensity decay is reintroduced and is applied for the first time to high frame-rate room-temperature data collection.
Authors:
; ; ;  [1] ;  [2] ; ;  [3] ;  [1] ;  [4] ;  [1]
  1. Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE (United Kingdom)
  2. Dectris Ltd, Neuenhofer Strasse 107, 5400 Baden (Switzerland)
  3. University of Oxford, The Henry Wellcome Building for Genomic Medicine, Roosevelt Drive, Oxford OX3 7BN (United Kingdom)
  4. (United Kingdom)
Publication Date:
OSTI Identifier:
22347797
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section D: Biological Crystallography; Journal Volume: 70; Journal Issue: Pt 5; Other Information: PMCID: PMC4014120; PMID: 24816094; PUBLISHER-ID: wa5063; OAI: oai:pubmedcentral.nih.gov:4014120; Copyright (c) Owen et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
Denmark
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLOGRAPHY; CRYSTALS; DECAY; DOSE RATES; PROTEINS; RADIATION EFFECTS