Enhanced-locality fiber-optic two-photon-fluorescence live-brain interrogation

Journal Article · Mon Feb 24 00:00:00 EST 2014 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.4864646· OSTI ID:22293087

Fedotov, I. V.; Doronina-Amitonova, L. V. ^[1]; Sidorov-Biryukov, D. A.; Fedotov, A. B. ^[1]; Anokhin, K. V. ^[2]; Kilin, S. Ya. ^[3]; Sakoda, K. ^[4]; Zheltikov, A. M. ^[1]

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1. International Laser Center, Physics Department, M.V. Lomonosov Moscow State University, Moscow 119992 (Russian Federation)
2. Kurchatov Institute National Research Center, Moscow (Russian Federation)
3. B.I. Stepanov Institute of Physics, National Academy of Sciences of Belarus, Minsk (Belarus)
4. National Institute for Materials Science, 1-1 Namiki, Tsukuba 305-0044 (Japan)

Two-photon excitation is shown to substantially enhance the locality of fiber-based optical interrogation of strongly scattering biotissues. In our experiments, a high-numerical-aperture, large-core-are fiber probe is used to deliver the 200-fs output of a 100-MHz mode-locked ytterbium fiber laser to samples of live mouse brain, induce two-photon fluorescence of nitrogen–vacancy centers in diamond markers in brain sample. Fiber probes with a high numerical aperture and a large core area are shown to enable locality enhancement in fiber-laser–fiber-probe two-photon brain excitation and interrogation without sacrificing the efficiency of fluorescence response collection.

• OSTI ID: 22293087
• Journal Information: Applied Physics Letters, Vol. 104, Issue 8; Other Information: (c) 2014 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
BRAIN
EXCITATION
FIBER OPTICS
FLUORESCENCE
LASERS
LOCALITY
MICE
MODE LOCKING
MULTI-PHOTON PROCESSES
NERVE TISSUE
NITROGEN
PHOTONS
SCATTERING
VACANCIES
YTTERBIUM