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Title: Structure and expression of a novel compact myelin protein – Small VCP-interacting protein (SVIP)

Highlights: •SVIP (small p97/VCP-interacting protein) co-localizes with myelin basic protein (MBP) in compact myelin. •We determined that SVIP is an intrinsically disordered protein (IDP). •The helical content of SVIP increases dramatically during its interaction with negatively charged lipid membrane. •This study provides structural insight into interactions between SVIP and myelin membranes. -- Abstract: SVIP (small p97/VCP-interacting protein) was initially identified as one of many cofactors regulating the valosin containing protein (VCP), an AAA+ ATPase involved in endoplasmic-reticulum-associated protein degradation (ERAD). Our previous study showed that SVIP is expressed exclusively in the nervous system. In the present study, SVIP and VCP were seen to be co-localized in neuronal cell bodies. Interestingly, we also observed that SVIP co-localizes with myelin basic protein (MBP) in compact myelin, where VCP was absent. Furthermore, using nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopic measurements, we determined that SVIP is an intrinsically disordered protein (IDP). However, upon binding to the surface of membranes containing a net negative charge, the helical content of SVIP increases dramatically. These findings provide structural insight into interactions between SVIP and myelin membranes.
Authors:
 [1] ;  [2] ;  [3] ;  [2] ;  [4] ;  [1] ;  [5]
  1. Department of Neurology, Vanderbilt University School of Medicine (United States)
  2. Department of Biochemistry, Vanderbilt University School of Medicine (United States)
  3. Center for Structural Biology, Vanderbilt University (United States)
  4. (United States)
  5. (TVHS) – Nashville VA (United States)
Publication Date:
OSTI Identifier:
22242137
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 440; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DICHROISM; ENDOPLASMIC RETICULUM; MYELIN; NERVOUS SYSTEM; NUCLEAR MAGNETIC RESONANCE