Bone Implant Interface Investigation by Synchrotron Radiation X-Ray Microfluorescence

Journal Article · Tue Apr 06 00:00:00 EDT 2010 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.3399631· OSTI ID:21371755

Calasans-Maia, M ^[1]; Sales, E; Lopes, R T ^[2]; Granjeiro, J M ^[3]; Lima, I ^[1]

---

1. Odondology Department, Fluminense Federal Univeristy, Niteroi 24030-900, RJ (Brazil)
2. Nuclear Instrumentation Laboratory-PEN/COPPE, Federal Univeristy of Rio de Janeiro, Rio de Janeiro 21941-914, RJ (Brazil)
3. Department of Cell and Molecular Biology, Fluminense Federal University, Niteroi 24030-900, RJ (Brazil)

Zinc is known to play a relevant role in growth and development; it has stimulatory effects on in vitro and in vivo bone formation and an inhibitory effect on in vitro osteoclastic bone resorption. The inorganic component of the bone tissue is nonstoichiometric apatite; changes in the composition of hidroxyapatite are subject of studies in order to improve the tissue response after implantation. The objective of this study was to investigate the effect of 0.5% zinc-containing hydroxyapatite in comparison to hydroxyapatite on osseous repair of rabbit's tibia. Cylinders (2x6 mm) of both materials were produced according to the specification of the International Organization for Standardization. Ethics Commission on Teaching and Research in Animals approved this project (HUAP-195/06). Fifteen White New Zealand rabbits were submitted to general anesthesia and two perforations (2 mm) were made in each tibia for implantation of zinc-containing hydroxyapatite cylinders (left tibia) and hydroxyapatite cylinders (right tibia). After 1, 2 and 4 weeks, the animals were killed and one fragment of each tibia with the cylinder was collected and embedded in a methacrylate-based resin and cut into slices (approx200 {mu}m thickness), parallel to the implant's long axis with a precision diamond saw for Synchrotron Radiation X-ray Microfluorescence investigation. The accomplishment of the standard procedures helped the planning, execution and the comparative analysis of the results. The chemical and physical properties of the biomaterials were modified after its implantation and the incorporation of zinc. Both materials are biocompatible and promote osteoconduction and favored bone repair.

• OSTI ID: 21371755
• Journal Information: AIP Conference Proceedings, Vol. 1221, Issue 1; Conference: 20. international congress on X-ray optics and microanalysis, Karlsruhe (Germany), 15-18 Sep 2009; Other Information: DOI: 10.1063/1.3399631; (c) 2010 American Institute of Physics; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
APATITES
BIOLOGICAL MATERIALS
BONE TISSUES
COMPARATIVE EVALUATIONS
IMPLANTS
IN VITRO
IN VIVO
INTERFACES
MULTI-ELEMENT ANALYSIS
QUANTITATIVE CHEMICAL ANALYSIS
RABBITS
SYNCHROTRON RADIATION
TIBIA
X-RAY FLUORESCENCE ANALYSIS
ZINC
ANIMAL TISSUES
ANIMALS
BODY
BREMSSTRAHLUNG
CHEMICAL ANALYSIS
CONNECTIVE TISSUE
ELECTROMAGNETIC RADIATION
ELEMENTS
EVALUATION
MAMMALS
MATERIALS
METALS
MINERALS
NONDESTRUCTIVE ANALYSIS
ORGANS
PHOSPHATE MINERALS
RADIATIONS
SKELETON
VERTEBRATES
X-RAY EMISSION ANALYSIS