Thrombomodulin exerts cytoprotective effect on low-dose UVB-irradiated HaCaT cells

Journal Article · Fri Dec 12 00:00:00 EST 2008 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2008.10.049· OSTI ID:21255788

Iwata, Masahiro ^[1]; Kawahara, Ko-ichi ^[2]; Kawabata, Hisashi ^[1]; Ito, Takashi ^[2]; Mera, Kentaro ^[1]; Biswas, Kamal Krishna ^[2]; Tancharoen, Salunya ^[3]; Higashi, Yuko ^[4]; Kikuchi, Kiyoshi; Hashiguchi, Teruto ^[5]

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1. Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan)
2. Laboratory of Vascular Medicine, Department of Cardiovascular and Respiratory Disorders Advanced Therapeutics, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan)
3. Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400 (Thailand)
4. Dept. of Dermatology, Kagoshima Univ. Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan)
5. Lab. of Vascular Medicine, Dept. of Cardiovascular and Respiratory Disorders Advanced Therapeutics, Kagoshima Univ. Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan)

Thrombomodulin (TM) is an endothelial cell surface anticoagulant glycoprotein that performs antimetastatic, angiogenic, adhesive, and anti-inflammatory functions in various tissues. It is also expressed in epidermal keratinocytes. We found that a physiological dose (10 mJ/cm{sup 2}) of mid-wavelength ultraviolet irradiation (UVB) significantly induced TM expression via the p38mitogen-activated protein kinase (MAPK)/cyclic AMP response element (CRE) signaling pathway in the epidermal keratinocyte cell line HaCaT; this shows that TM regulates the survival of HaCaT cells. SB203580, a p38MAPK inhibitor, significantly decreased TM expression and the viability of cells exposed to UVB. Furthermore, overexpression of TM markedly increased cell viability, and it was abrogated by TM small interfering RNA (siRNA), suggesting that TM may play an important role in exerting cytoprotective effect on epidermal keratinocytes against low-dose UVB.

• OSTI ID: 21255788
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 377, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2008.10.049; PII: S0006-291X(08)01999-2; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ADHESIVES
AMP
ANTICOAGULANTS
GLYCOPROTEINS
INFLAMMATION
IRRADIATION
RNA
ULTRAVIOLET RADIATION