Nuclear factor of activated T-cells (NFAT) plays a role in SV40 infection
- Graduate Program in Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912 (United States)
- Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912 (United States)
Recent evidence highlighted a role for the transcription factor, nuclear factor of activated T-cells (NFAT), in the transcription of the human polyomavirus JCV. Here we show that NFAT is also important in the transcriptional control of the related polyomavirus, Simian Virus 40 (SV40). Inhibition of NFAT activity reduced SV40 infection of Vero, 293A, and HeLa cells, and this block occurred at the stage of viral transcription. Both NFAT3 and NFAT4 bound to the SV40 promoter through {kappa}B sites located within the 72 bp repeated enhancer region. In Vero cells, NFAT was involved in late transcription, but in HeLa and 293A cells both early and late viral transcription required NFAT activity. SV40 large T-Ag was found to increase NFAT activity and provided a positive feedback loop to transactivate the SV40 promoter.
- OSTI ID:
- 21078025
- Journal Information:
- Virology, Vol. 372, Issue 1; Other Information: DOI: 10.1016/j.virol.2007.10.029; PII: S0042-6822(07)00694-0; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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