Functional role of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells
- Second Department of Internal Medicine, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543 (Japan)
- Department of Pharmacology, Akita University School of Medicine, Akita (Japan)
We investigated the functional role of STIM1, a Ca{sup 2+} sensor in the endoplasmic reticulum (ER) that regulates store-operated Ca{sup 2+} entry (SOCE), in vascular smooth muscle cells (VSMCs). STIM1 was mainly localized at the ER and plasma membrane. The knockdown of STIM1 expression by small interfering (si) RNA drastically decreased SOCE. In contrast, an EF-hand mutant of STIM1, STIM1{sup E87A}, produced a marked increase in SOCE, which was abolished by co-transfection with siRNA to transient receptor potential canonical 1 (TRPC1). In addition, transfection with siRNA against STIM1 suppressed phosphorylation of cAMP-responsive element binding protein (CREB) and cell growth. These results suggest that STIM1 is an essential component of SOCE and that it is involved in VSMC proliferation.
- OSTI ID:
- 21032911
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 361, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2007.07.096; PII: S0006-291X(07)01578-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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