Platelet-rich plasma stimulates osteoblastic differentiation in the presence of BMPs

Journal Article · Fri Sep 14 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.06.142· OSTI ID:20991528

Tomoyasu, Akihiro ^[1]; Higashio, Kanji ^[1]; Kanomata, Kazuhiro ^[1]; Goto, Masaaki ^[1]; Kodaira, Kunihiko ^[1]; Serizawa, Hiroko ^[1]; Suda, Tatsuo ^[1]; Nakamura, Atsushi; Nojima, Junya; Fukuda, Toru ^[1]; Katagiri, Takenobu ^[1]

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1. Division of Pathophysiology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka-shi, Saitama 350-1241 (Japan)

Platelet-rich plasma (PRP) is clinically used as an autologous blood product to stimulate bone formation in vivo. In the present study, we examined the effects of PRP on proliferation and osteoblast differentiation in vitro in the presence of bone morphogenetic proteins (BMPs). PRP and its soluble fraction stimulated osteoblastic differentiation of myoblasts and osteoblastic cells in the presence of BMP-2, BMP-4, BMP-6 or BMP-7. The soluble PRP fraction stimulated osteoblastic differentiation in 3D cultures using scaffolds made of collagen or hydroxyapatite. Moreover, heparin-binding fractions obtained from serum also stimulated osteoblastic differentiation in the presence of BMP-4. These results suggested that platelets contain not only growth factors for proliferation but also novel potentiator(s) for BMP-dependent osteoblastic differentiation.

• OSTI ID: 20991528
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 361, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2007.06.142; PII: S0006-291X(07)01399-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
APATITES
BLOOD PLASMA
CELL PROLIFERATION
COLLAGEN
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
HEPARIN
IN VITRO
IN VIVO
MYOBLASTS
SKELETON