Human Cementum Protein 1 induces expression of bone and cementum proteins by human gingival fibroblasts

Journal Article · Fri Jul 06 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2007.04.204· OSTI ID:20991426

Carmona-Rodriguez, Bruno ^[1]; Alvarez-Perez, Marco Antonio ^[1]; Narayanan, A Sampath ^[2]; Zeichner-David, Margarita ^[3]; Reyes-Gasga, Jose ^[4]; Molina-Guarneros, Juan ^[5]; Garcia-Hernandez, Ana Lilia ^[1]; Suarez-Franco, Jose Luis ^[1]; Chavarria, Ivet Gil ^[1]; Villarreal-Ramirez, Eduardo ^[1]; Arzate, Higinio ^[1]

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1. Laboratorio de Biologia Celular y Molecular, Facultad de Odontologia, UNAM, Cd. Universitaria, Coyoacan, Mexico, D.F. 04510 (Mexico)
2. Department of Pathology, School of Medicine, UW, Seattle (United States)
3. Center for Craniofacial Molecular Biology, School of Dentistry, USC, Los Angeles (United States)
4. Instituto de Fisica, UNAM (Mexico)
5. Facultad de Medicina, UNAM (Mexico)

We recently presented evidence showing that a human cementoblastoma-derived protein, named Cementum Protein 1 (CEMP1) may play a role as a local regulator of cementoblast differentiation and cementum-matrix mineralization. This protein was shown to be expressed by cementoblasts and progenitor cells localized in the periodontal ligament. In this study we demonstrate that transfection of CEMP1 into human gingival fibroblasts (HGF) induces mineralization and expression of bone and cementum-matrix proteins. The transfected HGF cells had higher alkaline phosphatase activity and proliferation rate and they expressed genes for alkaline phosphatase, bone sialoprotein, osteocalcin, osteopontin, the transcription factor Runx2/Cbfa1, and cementum attachment protein (CAP). They also produced biological-type hydroxyapatite. These findings indicate that the CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and that it might have a potential function in cementum and bone formation.

• OSTI ID: 20991426
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 358, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2007.04.204; PII: S0006-291X(07)00920-5; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ALKALINE PHOSPHATASE
APATITES
CELL PROLIFERATION
FIBROBLASTS
GENES
HUMAN POPULATIONS
LIGAMENTS
MATRICES
MINERALIZATION
SKELETON
TRANSCRIPTION FACTORS